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WDYHV1 antibody

Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298
Anti-WDYHV1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120298
Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298

Cat. No. GTX120298

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all WDYHV1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDYHV1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885479

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WDYHV motif containing 1 , C8orf32

Background

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.

Database

Research Area

DATA IMAGES

Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298

GTX120298 IHC-P Image

WDYHV1 antibody detects WDYHV1 protein at nucleus in human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human breast cancer.
WDYHV1 antibody (GTX120298) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WDYHV1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120298

GTX120298 ICC/IF Image

WDYHV1 antibody detects WDYHV1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WDYHV1 protein stained by WDYHV1 antibody (GTX120298) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-WDYHV1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120298

GTX120298 IHC-P Image

WDYHV1 antibody detects WDYHV1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
WDYHV1 antibody (GTX120298) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169