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DPPA4 antibody [N1C3]

Anti-DPPA4 antibody [N1C3] used in Western Blot (WB). GTX118569
Anti-DPPA4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118569
Anti-DPPA4 antibody [N1C3] used in Western Blot (WB). GTX118569
Anti-DPPA4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118569
Anti-DPPA4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118569

Cat. No. GTX118569

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DPPA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Human ESC , NT2D1

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DPPA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

developmental pluripotency associated 4 , 2410091M23Rik

Background

May play a role in maintaining cell pluripotentiality.

Database

Research Area

DATA IMAGES

Anti-DPPA4 antibody [N1C3] used in Western Blot (WB). GTX118569

GTX118569 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
12% SDS PAGE
GTX118569 diluted at 1:500

Anti-DPPA4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118569

GTX118569 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DPPA4(GTX118569) antibody at 1:400 dilution.

Anti-DPPA4 antibody [N1C3] used in Western Blot (WB). GTX118569

GTX118569 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
12% SDS PAGE
GTX118569 diluted at 1:500

Anti-DPPA4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118569

GTX118569 ICC/IF Image

DPPA4 antibody [N1C3] detects DPPA4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DPPA4 protein stained by DPPA4 antibody [N1C3] (GTX118569) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-DPPA4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118569

GTX118569 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using DPPA4(GTX118569) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169