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PPP2R3A antibody [C2C3], C-term

Anti-PPP2R3A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114655
Anti-PPP2R3A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114655
Anti-PPP2R3A antibody [C2C3], C-term used in Western Blot (WB). GTX114655
Anti-PPP2R3A antibody [C2C3], C-term used in Western Blot (WB). GTX114655
Anti-PPP2R3A antibody [C2C3], C-term used in Western Blot (WB). GTX114655

Cat. No. GTX114655

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PPP2R3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

NT2D1 , U87-MG

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PPP2R3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164754

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 2 regulatory subunit B''alpha , PPP2R3 , PR130 , PR72

Background

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPP2R3A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114655

GTX114655 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPP2R3A(GTX114655) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPP2R3A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114655

GTX114655 ICC/IF Image

PPP2R3A antibody [C2C3], C-term detects PPP2R3A protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PPP2R3A antibody [C2C3], C-term used in Western Blot (WB). GTX114655

GTX114655 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
B: U87-MG
5% SDS PAGE
GTX114655 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP2R3A antibody [C2C3], C-term used in Western Blot (WB). GTX114655

GTX114655 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PPP2R3A antibody [C2C3], C-term used in Western Blot (WB). GTX114655

GTX114655 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169