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PSPC1 antibody

Anti-PSPC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119744
Anti-PSPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119744
Anti-PSPC1 antibody used in Western Blot (WB). GTX119744
Anti-PSPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119744

Cat. No. GTX119744

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PSPC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSPC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885452

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paraspeckle component 1 , PSP1

Cellular Localization

Nucleus , nucleolus , Nucleus matrix , Cytoplasm

Background

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.

Database

Research Area

DATA IMAGES

Anti-PSPC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119744

GTX119744 ICC/IF Image

PSPC1 antibody detects PSPC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSPC1 protein stained by PSPC1 antibody (GTX119744) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PSPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119744

GTX119744 IHC-P Image

PSPC1 antibody detects PSPC1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
PSPC1 antibody (GTX119744) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSPC1 antibody used in Western Blot (WB). GTX119744

GTX119744 WB Image

PSPC1 antibody detects PSPC1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
PSPC1 antibody (GTX119744) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PSPC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119744

GTX119744 IHC-P Image

PSPC1 antibody detects PSPC1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
PSPC1 antibody (GTX119744) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169