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PPP3CB antibody [N1N3]

KO/KD Overexpression
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141
Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

Cat. No. GTX111141

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PPP3CB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Mouse brain , Molt-4 , HeLa , 293T , PPP3CB-transfected 293T

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP3CB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951361

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 3 catalytic subunit beta , CALNA2 , CALNB , CNA2 , PP2Bbeta

Background

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Database

Research Area

DATA IMAGES

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

KO/KD

Wild-type (WT) and TLE1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP3CB antibody [N1N3] (GTX111141) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111141 diluted at 1:5000

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111141 diluted at 1:1000

Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141

GTX111141 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using PPP3CB(GTX111141) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP3CB antibody [N1N3] (GTX111141) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169