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TRPV6 antibody

Anti-TRPV6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54869
Anti-TRPV6 antibody used in Western Blot (WB). GTX54869
Anti-TRPV6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54869

Cat. No. GTX54869

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TRPV6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 (Intracellular, C-terminus) of human TRPV6 (Accession  : Q9H1D0).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887903

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily V member 6 , ABP/ZF , CAT1 , CATL , ECAC2 , HRPTTN , HSA277909 , LP6728 , ZFAB

Cellular Localization

Cell membrane

Background

This gene encodes a member of a family of multipass membrane proteins that functions as calcium channels. The encoded protein contains N-terminal ankyrin repeats, which are required for channel assembly and regulation. Translation initiation for this protein occurs at a non-AUG start codon that is decoded as methionine. This gene is situated next to a closely related gene for transient receptor potential cation channel subfamily V member 5 (TRPV5). This locus has experienced positive selection in non-African populations, resulting in several non-synonymous codon differences among individuals of different genetic backgrounds. [provided by RefSeq, Feb 2015]

Database

Research Area

DATA IMAGES

Anti-TRPV6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54869

GTX54869 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX54869 TRPV6 antibody.
Panel A : TRPV6 channel (red) in DRG neurons.
Panel B : Staining with mouse anti-Parvalbumin (green) in the same DRG section.
Panel C : Merge of TRPV6 and Parvalbumin demonstrates colocalization (arrows).
Dilution : 1:100

Anti-TRPV6 antibody used in Western Blot (WB). GTX54869

GTX54869 WB Image

WB analysis of PC-3 cell lysate using GTX54869 TRPV6 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPV6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54869

GTX54869 ICC/IF Image

ICC/IF analysis of PFA-fixed PC-3 cells using GTX54869 TRPV6 antibody.
Panel A : Primary antibody
Panel B : Primary antibody prior incubated with immunogen peptide
Dilution : 1:20

REFERENCE

Application Reference

Sawatani T et al. Am J Physiol Cell Physiol 2019; (Epub) trpv2 channels mediate * secretion induced by cell swelling in mouse pancreatic β-cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

TRPV6 antibody Cat. No. GTX54869

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-TRPV6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54869
Date : Anonymous submitted on 24-Apr-2017
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Endometrium tissue
Blocking : 37°C, 1Hr
Fixation : Formalin
Permeabilization /Antigen retrieval : EDTA
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-TRPV6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54869
Date : Anonymous submitted on 24-Apr-2017
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Endometrium tissue
Blocking : 37°C, 1Hr
Fixation : Formalin
Permeabilization /Antigen retrieval : EDTA
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639