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WDR12 antibody [N1C2]

Anti-WDR12 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120474
Anti-WDR12 antibody [N1C2] used in Western Blot (WB). GTX120474
Anti-WDR12 antibody [N1C2] used in Western Blot (WB). GTX120474

Cat. No. GTX120474

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all WDR12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDR12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat domain 12 , YTM1

Cellular Localization

Nucleus , nucleolus , nucleoplasm

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein is highly similar to the mouse WD repeat domain 12 protein at the amino acid level. The protein encoded by this gene is a component of a nucleolar protein complex that affects maturation of the large ribosomal subunit.

Database

Research Area

DATA IMAGES

Anti-WDR12 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120474

GTX120474 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using WDR12(GTX120474) antibody at 1:200 dilution.

Anti-WDR12 antibody [N1C2] used in Western Blot (WB). GTX120474

GTX120474 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX120474 diluted at 1:1000

Anti-WDR12 antibody [N1C2] used in Western Blot (WB). GTX120474

GTX120474 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with WDR12 antibody [N1C2] (GTX120474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169