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IFT122 antibody [C1C2], Internal

Anti-IFT122 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119175
Anti-IFT122 antibody [C1C2], Internal used in Western Blot (WB). GTX119175
Anti-IFT122 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119175

Cat. No. GTX119175

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all IFT122 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IFT122. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623305

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intraflagellar transport 122 , CED , CED1 , SPG , WDR10 , WDR10p , WDR140

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IFT122 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119175

GTX119175 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using IFT122(GTX119175) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IFT122 antibody [C1C2], Internal used in Western Blot (WB). GTX119175

GTX119175 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX119175 diluted at 1:1000

Anti-IFT122 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119175

GTX119175 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using IFT122(GTX119175) antibody at 1:500 dilution.

REFERENCE

Application Reference

Radhakrishnan U et al. Platelets 2017; : 43110 Intraflagellar transport proteins are involved in thrombocyte filopodia formation and secretion.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169