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UTP6 antibody

Anti-UTP6 antibody used in Western Blot (WB). GTX106004
Anti-UTP6 antibody used in IHC (Paraffin sections) (IHC-P). GTX106004
Anti-UTP6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106004

Cat. No. GTX106004

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, NB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UTP6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
NB Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UTP6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036386

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UTP6 small subunit processome component , C17orf40 , HCA66

Cellular Localization

Nucleus , nucleolus

Background

Involved in nucleolar processing of pre-18S ribosomal RNA.

Database

DATA IMAGES

Anti-UTP6 antibody used in Western Blot (WB). GTX106004

GTX106004 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX106004 diluted at 1:1000

Anti-UTP6 antibody used in IHC (Paraffin sections) (IHC-P). GTX106004

GTX106004 IHC-P Image

UTP6 antibody detects UTP6 protein at nucleus on U251 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U251 xenograft.
UTP6 antibody (GTX106004) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UTP6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106004

GTX106004 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using UTP6(GTX106004) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

REFERENCE

Application Reference

Kumazawa T et al. Sci Rep 2015; 5 (Epub) : 10854 Gradual reduction in rRNA transcription triggers p53 acetylation and apoptosis via MYBBP1A.
Application : WB, NB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169