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PKC iota / lambda (phospho Thr563) antibody

Orthogonal
Anti-PKC iota / lambda (phospho Thr563) antibody used in Western Blot (WB). GTX25813

Cat. No. GTX25813

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Dog
Package
50 μl ($399)
See all PKC iota products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS 1:20
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Product Note

This antibody reacts with PKC lambda immunoprecipitates, indicating cross-reactivity for PKC lambda [pT563]. PKC zeta [pT56] (83% homologous) has been shown to cross-react by peptide competition. Peptide competition also suggests that this antibody may partially cross-react with PKC beta 1 [pS642] (58% homologous) and PKC nu [pT655] (42% homologous).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide derived from a region of human PKC iota that contains threonine 563.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_384130

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C iota , DXS1179E , PKCI , nPKC-iota

Cellular Localization

Cytoplasm,Membrane,Endosome,Nucleus

Background

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC iota / lambda (phospho Thr563) antibody used in Western Blot (WB). GTX25813

GTX25813 WB Image

Orthogonal

WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25813 PKC iota / lambda (phospho Thr563) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

Application Reference

Ahsan MK et al. J Clin Med 2022; 11 (14) Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID).
Forteza R et al. Mol Biol Cell 2016; 27 (14) : 2186-97 Conditional knockout of polarity complex (atypical) PKCι reveals an anti-inflammatory function mediated by NF-κB.
Kravtsov D et al. Am J Physiol Gastrointest Liver Physiol 2014; : ajpgi.00180.2014 Myosin 5b loss of function leads to defects in polarized signalling: Implication for Microvillus Inclusion Disease pathogenesis and treatment.
Application : WB
Reactivity : Human
Mashukova A et al. J Cell Sci 2014; (Epub) BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells under inflammatory signaling.
Application : WB, ICC/IF
Reactivity : Human
Forteza R et al. Biol Open 2013; 2 (11) : 1264-9 Par-complex aPKC and Par3 cross-talk with innate immunity NF-κB pathway in epithelial cells.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399