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ARHGAP15 antibody [N1N3]

Anti-ARHGAP15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119660
Anti-ARHGAP15 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119660
Anti-ARHGAP15 antibody [N1N3] used in Western Blot (WB). GTX119660

Cat. No. GTX119660

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Chicken
Package
100 μl ($399),
25 μl ($169)
See all ARHGAP15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARHGAP15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618823

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GTPase activating protein 15 , BM046

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

RHO GTPases (see ARHA; MIM 165390) regulate diverse biologic processes, and their activity is regulated by RHO GTPase-activating proteins (GAPs), such as ARHGAP15 (Seoh et al., 2003 [PubMed 12650940]).[supplied by OMIM]

Database

DATA IMAGES

Anti-ARHGAP15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119660

GTX119660 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using ARHGAP15(GTX119660) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARHGAP15 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119660

GTX119660 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ARHGAP15(GTX119660) antibody at 1:500 dilution.

Anti-ARHGAP15 antibody [N1N3] used in Western Blot (WB). GTX119660

GTX119660 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX119660 diluted at 1:3000

REFERENCE

Application Reference

Graziano BR et al. J Cell Biol 2017; A module for Rac temporal signal integration revealed with optogenetics.
Application : WB
Reactivity : Human
Kosla J et al. Gene 2013; 513 (1) : 90-100 Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts.
Application : WB
Reactivity : Chicken
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169