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MAPK4 antibody [N1C1]

Anti-MAPK4 antibody [N1C1] used in Western Blot (WB). GTX104128
Anti-MAPK4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX104128
Anti-MAPK4 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104128

Cat. No. GTX104128

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAPK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

H1299 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAPK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950876

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 4 , ERK-4 , ERK4 , PRKM4 , p63-MAPK , p63MAPK

Background

Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus where it phosphorylates nuclear targets. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAPK4 antibody [N1C1] used in Western Blot (WB). GTX104128

GTX104128 WB Image

Sample(30 ug whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX104128 diluted at 1:1000

Anti-MAPK4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX104128

GTX104128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAPK4(GTX104128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAPK4 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104128

GTX104128 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPK4(GTX104128) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169