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NDUFA12 antibody [N1C3]

Anti-NDUFA12 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109567
Anti-NDUFA12 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109567
Anti-NDUFA12 antibody [N1C3] used in Western Blot (WB). GTX109567
Anti-NDUFA12 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109567
Anti-NDUFA12 antibody [N1C3] used in Western Blot (WB). GTX109567

Cat. No. GTX109567

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NDUFA12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human NDUFA12 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950980

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit A12 , B17.2 , DAP13 , MC1DN23

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Database

Research Area

DATA IMAGES

Anti-NDUFA12 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109567

GTX109567 IHC-P Image

NDUFA12 antibody [N1C3] detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFA12 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109567

GTX109567 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12(GTX109567) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFA12 antibody [N1C3] used in Western Blot (WB). GTX109567

GTX109567 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFA12 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109567

GTX109567 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12(GTX109567) antibody at 1:200 dilution.

Anti-NDUFA12 antibody [N1C3] used in Western Blot (WB). GTX109567

GTX109567 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169