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NXF3 antibody [C1C3]

Anti-NXF3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116398
Anti-NXF3 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116398
Anti-NXF3 antibody [C1C3] used in Western Blot (WB). GTX116398

Cat. No. GTX116398

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NXF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NXF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632181

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear RNA export factor 3

Cellular Localization

Nucleus , Cytoplasm

Background

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NXF3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116398

GTX116398 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using NXF3(GTX116398) antibody at 1:200 dilution.

Anti-NXF3 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116398

GTX116398 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NXF3(GTX116398) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NXF3 antibody [C1C3] used in Western Blot (WB). GTX116398

GTX116398 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
C: U87-MG
D: MCF-7
7.5% SDS PAGE
GTX116398 diluted at 1:10000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169