APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
NT2D1 , IMR32 , U87-MG , MCF-7
Predict Reactivity
Mouse, Rat, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human PCDHA2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10632191
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protocadherin alpha 2 , PCDH-ALPHA2
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Background
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-PCDHA2 antibody [N1N2], N-term used in Western Blot (WB). GTX116525](/templates/default/js/lightbox/images/loading.gif "Anti-PCDHA2 antibody [N1N2], N-term used in Western Blot (WB). GTX116525")
|
GTX116525 WB Image
Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
C: U87-MG
D: MCF-7
7.5% SDS PAGE
GTX116525 diluted at 1:5000
|
REFERENCE
There are currently no references for PCDHA2 antibody [N1N2], N-term (GTX116525). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PCDHA2 antibody [N1N2], N-term (GTX116525). Be the first to share your experience with this product.
![Anti-PCDHA2 antibody [N1N2], N-term used in Western Blot (WB). GTX116525](/upload/website/prouct_img/small/GTX116525/GTX116525_WB_w_23060519_464.webp "Anti-PCDHA2 antibody [N1N2], N-term used in Western Blot (WB). GTX116525")
![Anti-PCDHA2 antibody [N1N2], N-term used in Western Blot (WB). GTX116525](/upload/website/prouct_img/normal/GTX116525/GTX116525_WB_w_23060519_464.webp "Anti-PCDHA2 antibody [N1N2], N-term used in Western Blot (WB). GTX116525")