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RNF23 antibody [N2C2], Internal

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637
Anti-RNF23 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116637
Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637
Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

Cat. No. GTX116637

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RNF23 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A431 , mouse testis , Rat testis

Predict Reactivity

Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618782

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 39 , RNF23 , TFP , TRIM39B

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. This gene lies within the major histocompatibility complex class I region on chromosome 6. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

GTX116637 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX116637 diluted at 1:1000

Anti-RNF23 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116637

GTX116637 IHC-P Image

RNF23 antibody [N2C2], Internal detects TRIM39 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
RNF23 antibody [N2C2], Internal (GTX116637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

GTX116637 WB Image

Sample (50 ug of whole cell lysate)
A: Rat testis
7.5% SDS PAGE
GTX116637 diluted at 1:1000

Anti-RNF23 antibody [N2C2], Internal used in Western Blot (WB). GTX116637

GTX116637 WB Image

Sample (50 ug of whole cell lysate)
A: mouse testis
7.5% SDS PAGE
GTX116637 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169