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EXOSC5 antibody

Orthogonal
Anti-EXOSC5 antibody used in Western Blot (WB). GTX118473
Anti-EXOSC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX118473
Anti-EXOSC5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118473

Cat. No. GTX118473

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EXOSC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human EXOSC5 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176191

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

exosome component 5 , RRP41B , RRP46 , Rrp46p , hRrp46p , p12B

Cellular Localization

Nucleus , nucleolus

Background

Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. Required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity.

Database

Research Area

DATA IMAGES

Anti-EXOSC5 antibody used in Western Blot (WB). GTX118473

GTX118473 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EXOSC5 antibody (GTX118473) diluted at 1:1000.

Anti-EXOSC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX118473

GTX118473 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EXOSC5(GTX118473) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EXOSC5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118473

GTX118473 ICC/IF Image

EXOSC5 antibody detects EXOSC5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EXOSC5 protein stained by EXOSC5 antibody (GTX118473) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Huang YH et al. Int J Biol Sci 2024; 20 (1) : 265-279 EXOSC5 maintains cancer stem cell activity in endometrial cancer by regulating the NTN4/integrin β1 signalling axis.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169