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CABC1 antibody [N3C2], Internal

Anti-CABC1 antibody [N3C2], Internal used in Western Blot (WB). GTX110374
Anti-CABC1 antibody [N3C2], Internal used in Western Blot (WB). GTX110374
Anti-CABC1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110374
Anti-CABC1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110374

Cat. No. GTX110374

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CABC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

IMR32 , Mouse heart

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CABC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721927

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coenzyme Q8A , ADCK3 , ARCA2 , CABC1 , COQ10D4 , COQ8 , SCAR9

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial protein similar to yeast ABC1, which functions in an electron-transferring membrane protein complex in the respiratory chain. It is not related to the family of ABC transporter proteins. Expression of this gene is induced by the tumor suppressor p53 and in response to DNA damage, and inhibiting its expression partially suppresses p53-induced apoptosis. Alternatively spliced transcript variants have been found; however, their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CABC1 antibody [N3C2], Internal used in Western Blot (WB). GTX110374

GTX110374 WB Image

Sample (50 μg of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX110374 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CABC1 antibody [N3C2], Internal used in Western Blot (WB). GTX110374

GTX110374 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX110374 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CABC1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110374

GTX110374 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CABC1(GTX110374) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CABC1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110374

GTX110374 ICC/IF Image

CABC1 antibody [N3C2], Internal detects CABC1 protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: CABC1 protein stained by CABC1 antibody [N3C2], Internal (GTX110374) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169