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NPD014 antibody

Anti-NPD014 antibody used in IHC (Paraffin sections) (IHC-P). GTX120166
Anti-NPD014 antibody used in Western Blot (WB). GTX120166
Anti-NPD014 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120166

Cat. No. GTX120166

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NPD014 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NPD014. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731612

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arginine and serine rich protein 1 , C1orf63 , NPD014

Database

Research Area

DATA IMAGES

Anti-NPD014 antibody used in IHC (Paraffin sections) (IHC-P). GTX120166

GTX120166 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using NPD014(GTX120166) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NPD014 antibody used in Western Blot (WB). GTX120166

GTX120166 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
12% SDS PAGE
GTX120166 diluted at 1:500

Anti-NPD014 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120166

GTX120166 ICC/IF Image

NPD014 antibody detects NPD014 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NPD014 protein stained by NPD014 antibody (GTX120166) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169