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CGN antibody

Anti-CGN antibody used in Western Blot (WB). GTX120159
Anti-CGN antibody used in IHC (Paraffin sections) (IHC-P). GTX120159
Anti-CGN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120159
Anti-CGN antibody used in Western Blot (WB). GTX120159

Cat. No. GTX120159

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all CGN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CGN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176830

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cingulin

Cellular Localization

Cell junction , tight junction

Background

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Database

Research Area

DATA IMAGES

Anti-CGN antibody used in Western Blot (WB). GTX120159

GTX120159 WB Image

Sample (30 ug of whole cell lysate)
A: Juakat
7.5% SDS PAGE
GTX120159 diluted at 1:500

Anti-CGN antibody used in IHC (Paraffin sections) (IHC-P). GTX120159

GTX120159 IHC-P Image

CGN antibody detects CGN protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. CGN antibody (GTX120159) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CGN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120159

GTX120159 ICC/IF Image

CGN antibody detects CGN protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CGN stained by CGN antibody (GTX120159) diluted at 1:500.

Anti-CGN antibody used in Western Blot (WB). GTX120159

GTX120159 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CGN antibody (GTX120159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169