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p400 antibody [N1], N-term

Anti-p400 antibody [N1], N-term used in Western Blot (WB). GTX116689
Anti-p400 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116689

Cat. No. GTX116689

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all p400 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

343 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p400. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630358

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E1A binding protein p400 , CAGH32 , P400 , TNRC12

Cellular Localization

Nucleus

Background

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity.

Database

Research Area

DATA IMAGES

Anti-p400 antibody [N1], N-term used in Western Blot (WB). GTX116689

GTX116689 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX116689 diluted at 1:1000

Anti-p400 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116689

GTX116689 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using p400(GTX116689) antibody at 1:200 dilution.

REFERENCE

Application Reference

Idrissou M et al. Cancer Genomics Proteomics 2020; 17 (6) : 687-694 TIP60/P400/H4K12ac Plays a Role as a Heterochromatin Back-up Skeleton in Breast Cancer.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169