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WDR19 antibody [N1N2], N-term

Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202
Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202
Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202
Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202

Cat. No. GTX119202

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all WDR19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WDR19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617362

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WD repeat domain 19 , ATD5 , CED4 , DYF-2 , IFT144 , NPHP13 , ORF26 , Oseg6 , PWDMP , SRTD5

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains six WD repeats, a clathrin heavy-chain repeat, and three transmembrane domains. This gene is conserved from C. elegans to human. It may participate in androgen-regulated signaling mechanisms or in the vesicular trafficking of androgen-regulated secretory processes. Alternatively spliced transcript variants encoding distinct isoforms have been reported but the full-length nature of one of these variants has not been defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202

GTX119202 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using WDR19(GTX119202) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202

GTX119202 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX119202 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-WDR19 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119202

GTX119202 IHC-P Image

WDR19 antibody [N1N2], N-term detects WDR19 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
WDR19 antibody [N1N2], N-term (GTX119202) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WDR19 antibody [N1N2], N-term used in Western Blot (WB). GTX119202

GTX119202 WB Image

WDR19 antibody detects WDR19 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WDR19 antibody (GTX119202) diluted by 1:1000.

REFERENCE

Application Reference

Liem KF Jr et al. J Cell Biol 2012; 197 (6) : 789-800 The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking.
Application : WB
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169