CYP4F11 antibody [N1N3]

Cat. No. GTX116701

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all CYP4F11 products

Datasheet
Document

View product citations for antibody GTX116701 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CYP4F11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172741

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 4 subfamily F member 11 , CYPIVF11

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein , Microsome membrane; Single-pass membrane protein , Endoplasmic reticulum membrane , Microsome membrane

Background

This gene, CYP4F11, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F2, is approximately 16 kb away. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 57834 CYP4F11
UniProt: Q9HBI6 CYP4F11

Research Area

DATA IMAGES

GTX116701 IHC-P Image

CYP4F11 antibody [N1N3] detects CYP4F11 protein at cytoplasm on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung adenocarcinoma.
CYP4F11 antibody [N1N3] (GTX116701) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116701 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX116701 diluted at 1:1000

GTX116701 ICC/IF Image

CYP4F11 antibody [N1N3] detects CYP4F11 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYP4F11 protein stained by CYP4F11 antibody [N1N3] (GTX116701) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty