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PTPRH antibody [C2C3], C-term

Anti-PTPRH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119064
Anti-PTPRH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX119064
Anti-PTPRH antibody [C2C3], C-term used in Western Blot (WB). GTX119064

Cat. No. GTX119064

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PTPRH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human PTPRH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618223

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type H , R-PTP-H , SAP1

Cellular Localization

Membrane; Single-pass type I membrane protein (Potential) , Cytoplasm

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PTPRH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119064

GTX119064 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PTPRH(GTX119064) antibody at 1:500 dilution.

Anti-PTPRH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX119064

GTX119064 IHC-P Image

PTPRH antibody [C2C3], C-term detects PTPRH protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
PTPRH antibody [C2C3], C-term (GTX119064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PTPRH antibody [C2C3], C-term used in Western Blot (WB). GTX119064

GTX119064 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX119064 diluted at 1:1000

REFERENCE

Application Reference

Chen FJ et al. J Asthma Allergy 2022; 15 : 133-144 PTPRH Alleviates Airway Obstruction and Th2 Inflammation in Asthma as a Protective Factor.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169