Rap1GAP antibody [N2C2], Internal

Cat. No. GTX113821

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX113821 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rap1GAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037840

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAP1 GTPase activating protein , RAP1GA1 , RAP1GAP1 , RAP1GAPII , RAPGAP

Cellular Localization

Golgi apparatus membrane; Peripheral membrane protein

Background

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

Database

Gene ID: 5909 RAP1GAP
UniProt: P47736 RAP1GAP

Research Area

DATA IMAGES

GTX113821 IHC-P Image

Rap1GAP antibody [N2C2], Internal detects Rap1GAP protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Rap1GAP antibody [N2C2], Internal (GTX113821) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113821 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rap1GAP antibody [N2C2], Internal (GTX113821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty