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RBM4 antibody

KO/KD
Anti-RBM4 antibody used in Western Blot (WB). GTX121565
Anti-RBM4 antibody used in Western Blot (WB). GTX121565
Anti-RBM4 antibody used in Western Blot (WB). GTX121565

Cat. No. GTX121565

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RBM4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa , 293T

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBM4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175358

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding motif protein 4 , LARK , RBM4A , ZCCHC21 , ZCRB3A

Cellular Localization

Nucleus , nucleolus , Cytoplasm

Background

May play a role in alternative splice site selection during pre-mRNA processing.

Database

Research Area

DATA IMAGES

Anti-RBM4 antibody used in Western Blot (WB). GTX121565

GTX121565 WB Image

KO/KD

Wild-type (WT) and RBM4 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBM4 antibody (GTX121565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RBM4 antibody used in Western Blot (WB). GTX121565

GTX121565 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RBM4 antibody (GTX121565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBM4 antibody used in Western Blot (WB). GTX121565

GTX121565 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX121565 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169