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RPL6 antibody

Anti-RPL6 antibody used in Western Blot (WB). GTX114913
Anti-RPL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114913
Anti-RPL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114913
Anti-RPL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114913
Anti-RPL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114913

Cat. No. GTX114913

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RPL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172828

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L6 , L6 , SHUJUN-2 , TAXREB107 , TXREB1

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6E family of ribosomal proteins. It is located in the cytoplasm. The protein can bind specifically to domain C of the tax-responsive enhancer element of human T-cell leukemia virus type 1, and it has been suggested that the protein may participate in tax-mediated transactivation of transcription. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL6 antibody used in Western Blot (WB). GTX114913

GTX114913 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
GTX114913 diluted at 1:1000

Anti-RPL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114913

GTX114913 IHC-P Image

RPL6 antibody detects RPL6 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
RPL6 antibody (GTX114913) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114913

GTX114913 IHC-P Image

RPL6 antibody detects RPL6 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
RPL6 antibody (GTX114913) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114913

GTX114913 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RPL6(GTX114913) antibody at 1:500 dilution.

Anti-RPL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX114913

GTX114913 IHC-P Image

RPL6 antibody detects RPL6 protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
RPL6 antibody (GTX114913) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Brody MJ et al. J Biol Chem 2019; (Epub) Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169