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RPL24 antibody

Anti-RPL24 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114729
Anti-RPL24 antibody used in IHC (Paraffin sections) (IHC-P). GTX114729
Anti-RPL24 antibody used in Western Blot (WB). GTX114729

Cat. No. GTX114729

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RPL24 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish, Bovine, Cat, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL24. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177278

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L24 , HEL-S-310 , L24

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPL24 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114729

GTX114729 ICC/IF Image

RPL24 antibody detects RPL24 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL24 stained by RPL24 antibody (GTX114729) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RPL24 antibody used in IHC (Paraffin sections) (IHC-P). GTX114729

GTX114729 IHC-P Image

RPL24 antibody detects RPL24 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RPL24 stained by RPL24 antibody (GTX114729) diluted at 1:500.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-RPL24 antibody used in Western Blot (WB). GTX114729

GTX114729 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RPL24 antibody (GTX114729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Roth F et al. Acta Neuropathol 2022; 144 (6) : 1157-1170 Assessment of PABPN1 nuclear inclusions on a large cohort of patients and in a human xenograft model of oculopharyngeal muscular dystrophy.
Xu B et al. BMC Genomics 2016; 17 : 25 Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169