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MRPL12 antibody [N1C3]

Anti-MRPL12 antibody [N1C3] used in Western Blot (WB). GTX114731
Anti-MRPL12 antibody [N1C3] used in Western Blot (WB). GTX114731
Anti-MRPL12 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114731
Anti-MRPL12 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114731

Cat. No. GTX114731

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MRPL12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Mouse brain , A431

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619823

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein L12 , 5c5-2 , L12mt , MRP-L31/34 , MRPL7 , MRPL7/L12 , RPML12

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRPL12 antibody [N1C3] used in Western Blot (WB). GTX114731

GTX114731 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114731 diluted at 1:1000

Anti-MRPL12 antibody [N1C3] used in Western Blot (WB). GTX114731

GTX114731 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MRPL12 antibody [N1C3] (GTX114731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MRPL12 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114731

GTX114731 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRPL12(GTX114731) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MRPL12 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114731

GTX114731 ICC/IF Image

MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (GTX114731) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Avolio R et al. bioRxiv 2023; Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Application : PLA
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169