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RPS15 antibody

Anti-RPS15 antibody used in Western Blot (WB). GTX101839
Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839
Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839
Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839
Anti-RPS15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101839
Anti-RPS15 antibody used in Western Blot (WB). GTX101839
Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839
Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839
Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839
Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839

Cat. No. GTX101839

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RPS15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722366

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S15 , RIG , S15

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPS15 antibody used in Western Blot (WB). GTX101839

GTX101839 WB Image

RPS15 antibody detects RPS15 protein by Western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
RPS15 antibody (GTX101839) dilution: 1:1000

Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839

GTX101839 IHC-P Image

RPS15 antibody detects RPS15 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
RPS15 antibody (GTX101839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839

GTX101839 IHC-P Image

RPS15 antibody detects RPS15 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
RPS15 antibody (GTX101839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS15 antibody used in IHC (Paraffin sections) (IHC-P). GTX101839

GTX101839 IHC-P Image

RPS15 antibody detects RPS15 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
RPS15 antibody (GTX101839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101839

GTX101839 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RPS15(GTX101839) antibody at 1:500 dilution.

Anti-RPS15 antibody used in Western Blot (WB). GTX101839

GTX101839 WB Image

RPS15 antibody detects RPS15 protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
RPS15 antibody (GTX101839) dilution: 1:1000

Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839

GTX101839 IHC-Wm Image

RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (GTX101839) dilution: 1:100.
Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839

GTX101839 IHC-Wm Image

RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (GTX101839) dilution: 1:100.
Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839

GTX101839 IHC-Wm Image

RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (GTX101839) dilution: 1:100.
Anti-RPS15 antibody used in IHC (Whole mount) (IHC-Wm). GTX101839

GTX101839 IHC-Wm Image

RPS15 antibody detects Rps15 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
RPS15 antibody (GTX101839) dilution: 1:100.

REFERENCE

Application Reference

Imai H et al. Nucleic Acids Res 2023; 51 (14) : e76 Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169