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Ryanodine Receptor antibody [34C]

Anti-Ryanodine Receptor antibody [34C] used in IHC (Paraffin sections) (IHC-P). GTX22868

Cat. No. GTX22868

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 34C

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Amphibians, Mustelid, Fish, Primate
Package
100 μl ($399)
See all Ryanodine Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5,000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:1,000
IP Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

565 kDa. ( Note )

Product Note

This antibody detects ryanodine receptor (RyR)-1 and RyR-2 isoforms. In chickens this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, it detects the alpha and beta isoforms and in fish it detects the alpha isoform.

PROPERTIES

Form

Liquid

Buffer

1x PBS pH7.4

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Partially purified chicken pectoral muscle ryanodine receptor.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384887

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ryanodine receptor 1 , CCO , MHS , MHS1 , PPP1R137 , RYDR , RYR , RYR-1 , SKRR

Background

This gene encodes a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ryanodine Receptor antibody [34C] used in IHC (Paraffin sections) (IHC-P). GTX22868

GTX22868 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX22868 Ryanodine Receptor antibody [34C].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

REFERENCE

Application Reference

Matsuki K et al. Pflugers Arch 2016; (Epub) Ryanodine receptor type 3 does not contribute to contractions in the mouse myometrium regardless of pregnancy.
Application : WB
Reactivity : Mouse

Clone Reference

Liu Z et al. Proc Natl Acad Sci U S A 2001; 98 (11):1535725 Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus.
Chan SL et al. J Biol Chem 2000; 275 (24):18195-200 Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons.
Yamada KA et al. J Cardiovasc Pharmacol 2000; 36 (1):14-21 Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399