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Xylosyltransferase II antibody [N2C1], Internal

Anti-Xylosyltransferase II antibody [N2C1], Internal used in Western Blot (WB). GTX110376
Anti-Xylosyltransferase II antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110376

Cat. No. GTX110376

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all XYLT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Japanese Medaka, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Xylosyltransferase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952617

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

xylosyltransferase 2 , PXYLT2 , SOS , XT-II , XT2 , xylT-II

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein , Golgi apparatus membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene is an isoform of xylosyltransferase, which belongs to a family of glycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein and initiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate, heparan sulfate, heparin and dermatan sulfate. The enzyme activity, which is increased in scleroderma patients, is a diagnostic marker for the determination of sclerotic activity in systemic sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Xylosyltransferase II antibody [N2C1], Internal used in Western Blot (WB). GTX110376

GTX110376 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX110376 diluted at 1:1000

Anti-Xylosyltransferase II antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110376

GTX110376 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Xylosyltransferase II(GTX110376) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

Application Reference

Matsuo K et al. J Biosci Bioeng 2018; (Epub) Xylosylation of proteins by expression of human xylosyltransferase 2 in plants.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169