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C17orf75 antibody [N1C1]

Anti-C17orf75 antibody [N1C1] used in Western Blot (WB). GTX108779
Anti-C17orf75 antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX108779
Anti-C17orf75 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX108779
Anti-C17orf75 antibody [N1C1] used in Western Blot (WB). GTX108779

Cat. No. GTX108779

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all C17orf75 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

mouse brain , U87-MG , SK-N-SH , IMR32 , SK-N-AS

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C17orf75. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949776

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromosome 17 open reading frame 75 , NJMU-R1 , SRI2

Background

May have a role in spermatogenesis.

Database

DATA IMAGES

Anti-C17orf75 antibody [N1C1] used in Western Blot (WB). GTX108779

GTX108779 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Njmu-R1 antibody [N1C1] (GTX108779) diluted at 1:3000.

Anti-C17orf75 antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX108779

GTX108779 IHC-Fr Image

Njmu-R1 antibody [N1C1] detects Njmu-R1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Njmu-R1 stained by Njmu-R1 antibody [N1C1] (GTX108779) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-C17orf75 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX108779

GTX108779 IHC-P Image

Njmu-R1 antibody [N1C1] detects Njmu-R1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Njmu-R1 antibody [N1C1] (GTX108779) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C17orf75 antibody [N1C1] used in Western Blot (WB). GTX108779

GTX108779 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Njmu-R1 antibody [N1C1] (GTX108779) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169