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MRPS5 antibody

KO/KD Orthogonal
Anti-MRPS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103930
Anti-MRPS5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103930
Anti-MRPS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103930
Anti-MRPS5 antibody used in Western Blot (WB). GTX103930

Cat. No. GTX103930

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MRPS5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Product Note

Knockdown/Knockout validation was supported by references (PMID:36949106).

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPS5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein S5 , MRP-S5 , S5mt

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRPS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103930

GTX103930 IHC-P Image

MRPS5 antibody detects MRPS5 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MRPS5 antibody (GTX103930) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MRPS5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103930

GTX103930 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MRPS5(GTX103930) antibody at 1:200 dilution.

Anti-MRPS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103930

GTX103930 IHC-P Image

MRPS5 antibody detects MRPS5 protein at cytosol on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
MRPS5 antibody (GTX103930) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MRPS5 antibody used in Western Blot (WB). GTX103930

GTX103930 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MRPS5 antibody (GTX103930) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Gao F et al. Nat Commun 2023; 14 (1) : 1595 A defect in mitochondrial protein translation influences mitonuclear communication in the heart.
Application : WB
Avolio R et al. bioRxiv 2023; Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Application : WB
Reactivity : Human
Pulman J et al. Hum Mol Genet 2018; (Epub) Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169