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VPS33A antibody [C1C3]

Orthogonal
Anti-VPS33A antibody [C1C3] used in Western Blot (WB). GTX119416
Anti-VPS33A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119416
Anti-VPS33A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119416
Anti-VPS33A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119416

Cat. No. GTX119416

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all VPS33A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human VPS33A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617835

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VPS33A core subunit of CORVET and HOPS complexes , MPSPS

Cellular Localization

Late endosome membrane; Peripheral membrane protein; Cytoplasmic side , Lysosome membrane; Peripheral membrane protein; Cytoplasmic side

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS33A antibody [C1C3] used in Western Blot (WB). GTX119416

GTX119416 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS33A antibody [C1C3] (GTX119416) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Anti-VPS33A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119416

GTX119416 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using VPS33A(GTX119416) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VPS33A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119416

GTX119416 ICC/IF Image

VPS33A antibody [C1C3] detects VPS33A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS33A protein stained by VPS33A antibody [C1C3] (GTX119416) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-VPS33A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119416

GTX119416 ICC/IF Image

Orthogonal

The data was published in the journal Biol Open in 2016.PMID: 27628032

REFERENCE

Application Reference

Guangyan Miao et al. Dev Cell 2021; 56 (4) : 427-442.e5 ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.
Application : WB
Uematsu M et al. Autophagy 2017; : 0 Accumulation of undegraded autophagosomes by expression of dominant-negative STX17 (syntaxin 17) mutants.
Application : WB
Reactivity : Human
Hubert V et al. Biol Open 2016; 5 (10) : 1516-1529 LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes.
Application : ICC/IF
Reactivity : Mouse
van der Kant R et al. J Biol Chem 2015; 290 (51) : 30280-90 Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
Jiang P et al. Mol Biol Cell 2014; 25 (8) : 1327-37 The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169