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GLUT3 antibody

Orthogonal Overexpression
Anti-GLUT3 antibody used in Western Blot (WB). GTX129175
Anti-GLUT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129175
Anti-GLUT3 antibody used in Western Blot (WB). GTX129175
Anti-GLUT3 antibody used in Western Blot (WB). GTX129175

Cat. No. GTX129175

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GLUT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC (Free Floating) Assay dependent

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

unboiled U87-MG membrane fraction extract , unboiled HepG2 membrane fraction extract , GLUT3-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human GLUT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885915

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 2 member 3 , GLUT3

Cellular Localization

Membrane; Multi-pass membrane protein

Database

Research Area

DATA IMAGES

Anti-GLUT3 antibody used in Western Blot (WB). GTX129175

GTX129175 WB Image

Overexpression

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:2000.

Anti-GLUT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129175

GTX129175 ICC/IF Image

GLUT3 antibody detects GLUT3 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GLUT3 stained by GLUT3 antibody (GTX129175) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GLUT3 antibody used in Western Blot (WB). GTX129175

GTX129175 WB Image

Orthogonal

Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

Anti-GLUT3 antibody used in Western Blot (WB). GTX129175

GTX129175 WB Image

Orthogonal

Unboiled U87-MG whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

REFERENCE

Application Reference

Chengkang He et al. JCI Insight 2022; 7 (5) Recurrent moderate hypoglycemia accelerates the progression of Alzheimer's disease through impairment of the TRPC6/GLUT3 pathway.
Application : IHC (Free Floating)
Reactivity : Mouse
He M et al. Oncogene 2019; (Epub) The miR-186-3p/EREG axis orchestrates tamoxifen resistance and aerobic glycolysis in breast cancer cells.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169