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SOS2 antibody

Anti-SOS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101723
Anti-SOS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101723
Anti-SOS2 antibody used in Western Blot (WB). GTX101723

Cat. No. GTX101723

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SOS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOS2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1241352

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SOS Ras/Rho guanine nucleotide exchange factor 2 , NS9 , SOS-2

Database

Research Area

DATA IMAGES

Anti-SOS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101723

GTX101723 IHC-P Image

SOS2 antibody detects SOS2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SOS2 antibody (GTX101723) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SOS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101723

GTX101723 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using SOS2(GTX101723) antibody at 1:500 dilution.

Anti-SOS2 antibody used in Western Blot (WB). GTX101723

GTX101723 WB Image

Sample (30 μg of whole cell lysate)
A: A549 5% SDS PAGE
GTX101723 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chu H et al. Anal Chem 2022; 94 (2) : 758-767 Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169