APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100 |
| 1:10-1:200 |
| 1:20-1:200 |
| 0.5-2 μg/test |
| Assay dependent |
|
Note :
ICC/IF
If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution.
IHC-P
If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits.
PROPERTIES
Form
Liquid
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified human erythrocyte beta-1 spectrin.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
spectrin beta, erythrocytic , EL3 , HS2 , HSPTB1 , SPH2
Cellular Localization
Cytoplasm, cytoskeleton
Background
This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
![Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808](/templates/default/js/lightbox/images/loading.gif "Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808")
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GTX22808 IHC-P Image
IHC-P analysis of human skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
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GTX22808 FACS Image
FACS analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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GTX22808 IHC-P Image
IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20
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GTX22808 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20
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GTX22808 FACS Image
FACS analysis of SH-SY5Y cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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GTX22808 FACS Image
FACS analysis of NIH/3T3 cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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GTX22808 ICC/IF Image
ICC/IF analysis of A431 cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
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GTX22808 ICC/IF Image
ICC/IF analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX22808 WB Image
WB analysis of 25 ug of Hela cell lysates using GTX22808 Spectrin beta 1 antibody [4C3].
Dilution : 1:1000
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REFERENCE
There are currently no references for Spectrin beta 1 antibody [4C3] (GTX22808). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Spectrin beta 1 antibody [4C3] (GTX22808). Be the first to share your experience with this product.
![Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_1143_IHC-P_w_23060620_404.webp "Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_151_FACS_w_23060620_707.webp "Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_1141_IHC-P_w_23060620_271.webp "Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_1142_IHC-P_w_23060620_561.webp "Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_153_FACS_w_23060620_801.webp "Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_152_FACS_w_23060620_116.webp "Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_485_ICC-IF_w_23060620_923.webp "Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_486_ICC-IF_w_23060620_410.webp "Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Western Blot (WB). GTX22808](/upload/website/prouct_img/small/GTX22808/GTX22808_1603_WB_w_23060620_160.webp "Anti-Spectrin beta 1 antibody [4C3] used in Western Blot (WB). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_1143_IHC-P_w_23060620_404.webp "Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_151_FACS_w_23060620_707.webp "Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_1141_IHC-P_w_23060620_271.webp "Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_1142_IHC-P_w_23060620_561.webp "Anti-Spectrin beta 1 antibody [4C3] used in IHC (Paraffin sections) (IHC-P). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_153_FACS_w_23060620_801.webp "Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_152_FACS_w_23060620_116.webp "Anti-Spectrin beta 1 antibody [4C3] used in Flow cytometry (FACS). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_485_ICC-IF_w_23060620_923.webp "Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_486_ICC-IF_w_23060620_410.webp "Anti-Spectrin beta 1 antibody [4C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22808")
![Anti-Spectrin beta 1 antibody [4C3] used in Western Blot (WB). GTX22808](/upload/website/prouct_img/normal/GTX22808/GTX22808_1603_WB_w_23060620_160.webp "Anti-Spectrin beta 1 antibody [4C3] used in Western Blot (WB). GTX22808")