GeneTex

  • Country / Location Selection

United States (US)

BRDT antibody [C3], C-term

Anti-BRDT antibody [C3], C-term used in Western Blot (WB). GTX100201

Cat. No. GTX100201

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all BRDT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

mouse testis

Predict Reactivity

Human, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BRDT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240521

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain testis associated , BRD6 , CT9 , SPGF21

Cellular Localization

Nucleus

Background

BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BRDT antibody [C3], C-term used in Western Blot (WB). GTX100201

GTX100201 WB Image

BRDT antibody detects BRDT protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with BRDT antibody (GTX100201) at a dilution of 1:500.

REFERENCE

Application Reference

Kim CR et al. Cell Rep 2020; 32 (4) : 107950 PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for BRDT antibody [C3], C-term (GTX100201). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169