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LOK antibody [N1N2], N-term

KO/KD
Anti-LOK antibody [N1N2], N-term used in Western Blot (WB). GTX111951
Anti-LOK antibody [N1N2], N-term used in Western Blot (WB). GTX111951
Anti-LOK antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111951
Anti-LOK antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111951

Cat. No. GTX111951

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LOK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LOK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723130

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 10 , LOK , PRO2729

Background

This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LOK antibody [N1N2], N-term used in Western Blot (WB). GTX111951

GTX111951 WB Image

KO/KD

Wild-type (WT) and LOK knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LOK antibody [N1N2], N-term (GTX111951) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LOK antibody [N1N2], N-term used in Western Blot (WB). GTX111951

GTX111951 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LOK antibody [N1N2], N-term (GTX111951) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LOK antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111951

GTX111951 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LOK(GTX111951) antibody at 1:500 dilution.

Anti-LOK antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111951

GTX111951 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using LOK(GTX111951) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169