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TCF7 antibody

Anti-TCF7 antibody used in IHC (Paraffin sections) (IHC-P). GTX113851
Anti-TCF7 antibody used in Immunoprecipitation (IP). GTX113851
Anti-TCF7 antibody used in Western Blot (WB). GTX113851
Anti-TCF7 antibody used in IHC (Paraffin sections) (IHC-P). GTX113851
Anti-TCF7 antibody used in Western Blot (WB). GTX113851

Cat. No. GTX113851

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TCF7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCF7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor 7 , TCF-1

Cellular Localization

Nucleus

Background

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

Database

Research Area

DATA IMAGES

Anti-TCF7 antibody used in IHC (Paraffin sections) (IHC-P). GTX113851

GTX113851 IHC-P Image

TCF7 antibody detects TCF7 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
TCF7 stained by TCF7 antibody (GTX113851) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TCF7 antibody used in Immunoprecipitation (IP). GTX113851

GTX113851 IP Image

Immunoprecipitation of TCF7 protein from Jurkat whole cell extracts using 5 μg of TCF7 antibody (GTX113851).
Western blot analysis was performed using TCF7 antibody (GTX113851).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TCF7 antibody used in Western Blot (WB). GTX113851

GTX113851 WB Image

TCF7 antibody detects TCF7 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
10 % SDS-PAGE
TCF7 antibody (GTX113851) dilution: 1:1000

Anti-TCF7 antibody used in IHC (Paraffin sections) (IHC-P). GTX113851

GTX113851 IHC-P Image

TCF7 antibody detects TCF7 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
TCF7 stained by TCF7 antibody (GTX113851) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TCF7 antibody used in Western Blot (WB). GTX113851

GTX113851 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TCF7 antibody (GTX113851) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169