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TLE2 antibody [C2C3], C-term

Anti-TLE2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106107
Anti-TLE2 antibody [C2C3], C-term used in Western Blot (WB). GTX106107

Cat. No. GTX106107

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TLE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TLE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952263

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TLE family member 2, transcriptional corepressor , ESG , ESG2 , GRG2

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-TLE2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106107

GTX106107 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TLE2(GTX106107) antibody at 1:500 dilution.

Anti-TLE2 antibody [C2C3], C-term used in Western Blot (WB). GTX106107

GTX106107 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX106107 diluted at 1:1500

REFERENCE

Application Reference

Xu Y et al. J Biol Chem 2023; 299 (6) : 104802 Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169