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TLE4 antibody [C1C3]

Anti-TLE4 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114842
Anti-TLE4 antibody [C1C3] used in Western Blot (WB). GTX114842
Anti-TLE4 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114842

Cat. No. GTX114842

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all TLE4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620169

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TLE family member 4, transcriptional corepressor , BCE-1 , BCE1 , E(spI) , E(spl) , ESG , ESG4 , GRG4 , Grg-4

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-TLE4 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114842

GTX114842 ICC/IF Image

TLE4 antibody [C1C3] detects TLE4 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TLE4 stained by TLE4 antibody [C1C3] (GTX114842) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TLE4 antibody [C1C3] used in Western Blot (WB). GTX114842

GTX114842 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX114842 diluted at 1:1000

Anti-TLE4 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114842

GTX114842 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TLE4(GTX114842) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169