Troponin T1 (slow) skeletal muscle antibody

Name: Troponin T1 (slow) skeletal muscle antibody
Brand: GeneTex
SKU: GTX109585
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Troponin T1 (slow) skeletal muscle antibody used in Western Blot (WB). GTX109585

Anti-Troponin T1 (slow) skeletal muscle antibody used in IHC (Paraffin sections) (IHC-P). GTX109585

Cat. No. GTX109585

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Pig
Package	100 μl ($399), 25 μl ($169)

See all Troponin T1 products

View product citations for antibody GTX109585 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Troponin T1 (slow) skeletal muscle Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952333

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

troponin T1, slow skeletal type , ANM , NEM5 , STNT , TNT , TNTS

Background

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 7138 TNNT1
UniProt: P13805 TNNT1

Research Area

DATA IMAGES

GTX109585 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX109585 diluted at 1:1000

GTX109585 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Porcine skeletal muscle, using Troponin T1(GTX109585) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109585 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Troponin T1 (slow skeletal)(GTX109585) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Megumi Yumoto et al. In Vivo 2022; 36 (2) : 563-569 Analysis of Masticatory Muscle Tendon-aponeurosis Hyperplasia by Using Next-generation Sequencing.

Application : WB

Reactivity : Human

Zhang T et al. Geroscience 2022; (Epub) Cardiac troponin T and autoimmunity in skeletal muscle aging.

Submit a Reference

REVIEW