Topoisomerase II beta antibody [C3], C-term

Cat. No. GTX102640

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Topoisomerase II beta products

View product citations for antibody GTX102640 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

Jurkat , K562 , THP-1

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Topoisomerase II beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169314

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA topoisomerase II beta , TOPIIB , top2beta

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. [provided by RefSeq]

Database

Gene ID: 7155 TOP2B
UniProt: Q02880 TOP2B

Research Area

DATA IMAGES

GTX102640 IP Image

Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 μg of Topoisomerase II beta antibody (GTX102640) 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody (GTX102640) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102640 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Topoisomerase II beta(GTX102640) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102640 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102640 ICC/IF Image

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

GTX102640 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
5% SDS PAGE
GTX102640 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102640 IHC-P Image

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102640 IHC-P Image

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102640 IHC-P Image

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102640 WB Image

The data was published in the journal Biomolecules in 2019. PMID: 31505885

REFERENCE

Application Reference

Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.

Application : WB

Reactivity : Human

Liu TP et al. R Soc Open Sci 2018; 5 (11) : 181321 Systematic polypharmacology and drug repurposing via an integrated L1000-based Connectivity Map database mining.

Application : WB

Reactivity : Human

Hangas A et al. Nucleic Acids Res 2018; (Epub)

Application : WB

Reactivity : Mouse

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REVIEW

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Application Reference

Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.

Application : WB

Reactivity : Human

Liu TP et al. R Soc Open Sci 2018; 5 (11):181321 Systematic polypharmacology and drug repurposing via an integrated L1000-based Connectivity Map database mining.

Application : WB

Reactivity : Human

Hangas A et al. Nucleic Acids Res 2018; (Epub)

Application : WB

Reactivity : Mouse

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Topoisomerase II beta antibody [C3], C-term

Cat. No. GTX102640

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX102640 IP Image

GTX102640 IHC-P Image

GTX102640 WB Image

GTX102640 ICC/IF Image

GTX102640 WB Image

GTX102640 IHC-P Image

GTX102640 IHC-P Image

GTX102640 IHC-P Image

GTX102640 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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