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TPR antibody

Anti-TPR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130142
Anti-TPR antibody used in Western Blot (WB). GTX130142

Cat. No. GTX130142

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TPR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

267 kDa. ( Note )

Positive Control

mouse lung

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TPR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886191

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

translocated promoter region, nuclear basket protein

Cellular Localization

Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TPR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130142

GTX130142 ICC/IF Image

TPR antibody detects TPR protein at nuclear envelope and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TPR protein stained by TPR antibody (GTX130142) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-TPR antibody used in Western Blot (WB). GTX130142

GTX130142 WB Image

TPR antibody detects TPR protein by western blot analysis. Mouse tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with TPR antibody (GTX130142) at a dilution of 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169