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XRCC3 antibody

Anti-XRCC3 antibody used in Western Blot (WB). GTX12050
Anti-XRCC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12050

Cat. No. GTX12050

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μg ($449)
See all XRCC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse XRCC3(290-305aa NQLLMRLMVDRTHEDD), identical to the related rat sequence.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair complementing defective repair in Chinese hamster cells 3

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion matrix

Background

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. Allelic variants in the human gene are associated with susceptibility to breast cancer and cutaneous malignant melanoma. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-XRCC3 antibody used in Western Blot (WB). GTX12050

GTX12050 WB Image

WB analysis of mouse brain tissue lysate using GTX12050 XRCC3 antibody.
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-XRCC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12050

GTX12050 IHC-P Image

IHC-P analysis of rat brain tissue using GTX12050 XRCC3 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449