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ZIC3 antibody [N3C3]

Anti-ZIC3 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX109987
Anti-ZIC3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109987
Anti-ZIC3 antibody [N3C3] used in Western Blot (WB). GTX109987

Cat. No. GTX109987

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ZIC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZIC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952655

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Zic family member 3 , HTX , HTX1 , VACTERLX , ZNF203

Background

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZIC3 antibody [N3C3] used in IHC (Frozen sections) (IHC-Fr). GTX109987

GTX109987 IHC-Fr Image

ZIC3 antibody [N3C3] detects ZIC3 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: ZIC3 protein stained by ZIC3 antibody [N3C3] (GTX109987) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-ZIC3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109987

GTX109987 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using ZIC3(GTX109987) antibody at 1:100 dilution.

Anti-ZIC3 antibody [N3C3] used in Western Blot (WB). GTX109987

GTX109987 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX109987 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169