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C21orf2 antibody [N1C2]

Anti-C21orf2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119046
Anti-C21orf2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119046
Anti-C21orf2 antibody [N1C2] used in Western Blot (WB). GTX119046

Cat. No. GTX119046

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all C21orf2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C21orf2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617370

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cilia and flagella associated protein 410 , C21orf2 , LRRC76 , RDMS , SMDAX , YF5/A2

Database

  • Gene ID: 755 CFAP410
  • UniProt: O43822 CFAP410

Research Area

DATA IMAGES

Anti-C21orf2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119046

GTX119046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using C21orf2(GTX119046) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C21orf2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119046

GTX119046 ICC/IF Image

C21orf2 antibody [N1C2] detects C21orf2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C21orf2 stained by C21orf2 antibody [N1C2] (GTX119046) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-C21orf2 antibody [N1C2] used in Western Blot (WB). GTX119046

GTX119046 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX119046 diluted at 1:500

REFERENCE

Application Reference

Wheway G et al. Nat Cell Biol 2015; 17 (8) : 1074-87 An siRNA-based functional genomics screen for the-identification of regulators of ciliogenesis and ciliopathy genes.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169