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ZNF133 antibody [N1C1-2]

Anti-ZNF133 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX114896
Anti-ZNF133 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX114896
Anti-ZNF133 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114896
Anti-ZNF133 antibody [N1C1-2] used in Western Blot (WB). GTX114896

Cat. No. GTX114896

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZNF133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF133. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620176

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 133 , ZNF150 , pHZ-13 , pHZ-66

Cellular Localization

Nucleus

Background

May be involved in transcriptional regulation as a repressor.

Database

DATA IMAGES

Anti-ZNF133 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX114896

GTX114896 IHC-P Image

ZNF133 antibody [N1C1-2] detects ZNF133 protein at nucleus in human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human breast cancer.
ZNF133 antibody [N1C1-2] (GTX114896) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZNF133 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX114896

GTX114896 IHC-P Image

ZNF133 antibody [N1C1-2] detects ZNF133 protein at nucleus in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
ZNF133 antibody [N1C1-2] (GTX114896) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZNF133 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114896

GTX114896 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF133(GTX114896) antibody at 1:200 dilution.

Anti-ZNF133 antibody [N1C1-2] used in Western Blot (WB). GTX114896

GTX114896 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 (GTX27909)
7.5% SDS PAGE
GTX114896 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169